A. Maize apices containing five leaf primordia fail to initiate any new leaves when cultured for seven days in 35 mM NPA. A total of five leaves were present after seven days in culture.

B. In contrast, dissected apices containing five leaf primordia develop eight additional leaves when cultured for seven days in the absence of NPA. L = Leaf#, P = plastochron# as described. [from Scanlon et al., 1996; Timmermans et al., 1998; Muehlbauer and Scanlon, unpublished; Schneeberger et al., 1998]

Light micrograph of a transverse section of a maize seedling (left) shows laser capture of a single-cell layered epidermis from the cleoptile (right) - From Nakozono et al.

SAM cross section